P-77: Follicular Fluid Concentrations of Interleukin-6, Interleukin-8, TNF-α and Interleukin 10 in Polycystic Ovarian Syndrome Women

نویسندگان

  • I Amiri Department of Obstetrics and Gynecology, Faculty of Medicine, Iran University of Medical Science, Tehran, Iran
  • M Ashrafi Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  • M Mehdizadeh Cellular and Molecular Research Center, Faculty of Advanced Technology in Medicine, Anatomy department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • M Saidijam Research Center for Molecular Medicine, , Hamadan University of Medical Sciences, Hamadan, Iran
  • R Aflatoonian Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  • T Artimani Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
چکیده مقاله:

a:4:{s:10:"Background";s:504:"While the etiology of PCOS has continued to remain a mystery, there can be found some evidence in support of the presence of chronic low-grade inflammation in women with this syndrome. One of the most significant manifestations of chronic inflammation is Pro-inflammatory cytokines. Therefore, the aim of this prospective study was to investigate the pattern of proinflammatory and anti-inflammatory cytokines in the follicular fluid (FF) of PCOS women undergoing in vitro fertilization (IVF).";s:19:"MaterialsAndMethods";s:741:"Eighty women who met the selection criteria were recruited, and the FF was obtained during the follicular aspiration. Hence, we classify them as follows: (A) PCOS women; (B) Control women with normal ovulatory function who underwent IVF for treatment of tubal and/ or male infertility. Obtained FF from each patient was centrifuged at 300 g for 10 min. The supernatant was used to determine the concentration of a quantification of pro inflammatory cytokines interleukin (IL)-8 , IL-6, IL-10, and tumor necrosis factor-alpha (TNF-α) which was measured by the sandwich enzyme immunoassay ELISA based on a monoclonal antibody specific for them (Boster Biological Technology ltd, USA), according to the manufacturer's instructions.";s:7:"Results";s:540:"After BMI was controlled, there was a significant increment in the concentration of inflammatory cytokines IL-8 (187 ± 43.01 pg/ml), IL-6 (29.39 ± 2.75 pg/ml) and TNF-α (6.35 ±1.7 pg/ml) in the FF of PCOS women undergoing controlled ovarian hyperstimulation versus control women(141.92 ± 28.87, 7.90 ± 0.28 and 4.02 ± 0.94 pg/ml respectively, P

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عنوان ژورنال

دوره 9  شماره 2

صفحات  75- 76

تاریخ انتشار 2015-09-01

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